By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols deals starting and skilled researchers the main entire selection of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the subjects diversity from the upkeep of bacterial tradition collections to points of the metabolism and body structure of remodeled tissues and transgenic vegetation. Drawing at the paintings of top scientists from laboratories all over the world, Agrobacterium Protocols offers a wealth of thoughts for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered vegetation. Its special techniques will facilitate speedy move of complicated options to different laboratories and their exploitation in basic and utilized plant biology.
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Additional resources for Agrobacterium Protocols (Methods in Molecular Biology Vol 44)
172, 1814-1822. 13 Rogowsky, P. M , Powell, B. , Zyprran, E. M , Steck, T. , and Kado, C. 63-kbp cloned as a single unit. Plasmtd 23,85-106. 14. Stachel, S. E. and Zambryski, P. (1986) virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens. Cell 46,325-333 CHAPTER6 Binary Ti Plasmid Gynheung Vectors An 1. Introduction Living organisms have been continuously evolving by assimilating new genetic material from the environment. However, this progress is very slow and often limited to transfer of genetic materials among closely related species.
Stachel, S E and Zambryski, P (1986) virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens. Cell 46,325-333 10. Douglas, C. , and Nester, E. W. (1982)Agrobacterium tumefaciens mutants affected m attachment to plant cells. J. Bacterial. 152, 1265-1275. 11. An, G , Ebert, P. , and Ha, S B (1988) Binary vectors, m Plant Molecular Biology Manual (Gelvin, S. B. and Schilperoort, R. ), Kluwer, Dordrecht, Netherlands, pp A3 1-19. 12. Lichtenstem, C P and Fuller, S.
5. Cultures in Erlenmeyer flasks are incubated at 27°C with shaking for 6 d. ODsoois measured every 12 h. At the end of the experiment, the culture is centrifuged and pH of the supernatant is noted (see Note 5). A typical result obtained using this protocol is shown in Fig. 1. Exposure to acetosyringone results in a 2-d delay in onset of growth, as compared to the control culture. Following this delay, optical density in the acetosyringone-treated culture increases at a rate similar to what had been observed for the control culture (Fig.