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Download Advances in Microbial Physiology, Vol. 20 by A. H. & Morris, J. Gareth [eds.] Rose PDF

By A. H. & Morris, J. Gareth [eds.] Rose

This quantity in a research-level sequence covers assorted points of microbial body structure and biochemistry together with inositol metabolisms in yeasts, bacterial adhesion, natural acids, the bacterial flagellum and the mechanical behaviour of bacterial mobile partitions. it really is meant to be of use to microbiologists, biochemists and biotechnologists. different comparable works during this sequence are volumes 29, 30 and 31.

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5-38 x lo6. Most recently, we (R. H. Lau and W. F. Doolittle, in preparation) have shown that this plasmid occurs in two strains of A. nidulanr and in the closely related Synechococcus cedrorum. The strains differ, however, in their content of a previously undescribed 5 x lo6 dalton plasmid, which appears to have been lost from one of the strains of A. nidulans. Roberts and Koths (1976), in the most thorough study published to date, showed that Agmenellum quadruplicatum (strain PR-6) harbours at least six CCC DNAs, which together comprise about 5 per cent of the total DNA.

The RNA products appear identical in size and sequence, if not in relative amounts, to those of the homologous enzyme. S. S. Miller and L. Bogorad (in preparation) have recently purified an RNA polymerase from Fremyella diplosiphon (a filamentous rivularian species). It shows five subunits of molecular weights 161, 134,91, 72 and 41 x lo3. Loss of the smallest polypeptide in terminal purification steps results in a four-fold reduction in specific activity. Antisera directed against RNA polymerases from E.

Both precursor and mature forms of A . nidulans 5 s have been “fingerprinted” (by twodimensional separation of the products of T1 ribonuclease digestion). , 1974a). The kinetics of p5 labelling in viuo suggest that there is no significant pool of precursor molecules larger than p5. Thus 5 s maturation in A . nidulans appears very similar to the homologous process in the Gram-negative bacteria E. , 1973). Maturation of all A . , 1974). 68 x lo6 daltons) have been reported for Toolypothrix distorta, a filamentous cyanobacterium (Grierson and Smith, 1973).

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